1. First Steps: Unpacking and installation of the Instrument. Tip: Add 0.001 to 0.005% of NaN3 to the water bottle to avoid growth of microorganisms. Read manuals carefully.
2. First Measurement: Analysis of fresh and inactivated pollen to detect the area of viable pollen. The inactivation step serves as a control and is normally not needed. Tip: Do not touch the golden contact pins.
3. Sample preparation: Use collected pollen, anthers or crushed buds in AmphaFluid buffer and filter before the measurement. Tip: Make sure re-used filters are really clean and dry.
4. In-Field Measurement: Ampha Z30 is designed for use in the lab and in the field. For mobile use it can be powered by car battery or rechargeable batteries. Tip: Remove chip and disconnect all external cables before moving the instrument.
5. Reuse Samples: The label-free measurement enables reuse of samples after measurement. Examples for potential applications are time studies, method validation and subsequent analysis with other methods.
6. Daily Maintenance: Never forget to run the software guided wash cycle with AmphaClean at least once a day after the last experiment in order to keep the tubes and sensors clean. Tip: Empty the waste bottle every day.
7. Unblock Chip: Chips may clog when measuring samples with too high concentrations. They can be unblocked with the chip wash station. Tip: Avoid sedimentation of pollen by shaking the sample immediately before measurement.
8. Set Gating: After the measurement you can analyze the pollen populations by software supported counting of cell populations ("gating").Tip: Use ESC to exit the gating mode.
9. Generate Template: The settings for the experiment and the subsequent automatic analysis (including gating) can be stored in a template for serial analysis.
10. Work with Template: Work with templates when using pre-defined experimental settings and automated gating analysis.
11. Generate Report: There are Excel and HTML reports for easy documentation and further use.